EFFECT OF UKRAINIAN-REGISTERED PROBIOTIC PREPARATIONS ON THE TAXONOMIC STRUCTURE OF THE INTESTINAL MICROBIOME, PRODUCTIVITY, AND VITAMIN STATUS OF COBB 500 BROILER CHICKENS
DOI:
https://doi.org/10.37000/abbsl.2025.117.06Keywords:
broiler chickens, intestinal microbiome, probiotics, Bacillus subtilis, Saccharomyces cerevisiae, productivity, vitamins, Firmicutes.Abstract
The study aimed to investigate the effect of Ukrainian-registered probiotic preparations on the quantitative and qualitative composition of intestinal microbiota, productivity, and vitamin status of Cobb 500 broiler chickens. The experiment involved three groups: control (basic diet), group II (with yeast probiotic ProbioFerm-D), and group III (with bacterial probiotic Cellobacterin-T based on Bacillus subtilis). Probiotic supplementation significantly increased the total bacterial population in the ceca compared to control: 1.71 times with ProbioFerm-D and 2.84 times with Cellobacterin-T (P<0.05). The dominant taxa included Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria. A reduction in Shannon diversity index was observed in the probiotic groups, indicating stabilization of the microbial consortium due to colonization by probiotic strains. The most pronounced effect was observed with Cellobacterin-T, which promoted active colonization of the gut by Bacillus subtilis and increased Lactobacillus abundance by 47.8% (P<0.005). Both probiotics reduced opportunistic Enterobacteriaceae (by 35.0–45.9%) and Campylobacteraceae (threefold reduction with Cellobacterin-T, P<0.005). Growth performance improved significantly: live weight at 37 days was 1.6% higher in group II and 4.4% higher in group III (P≤0.01) compared to control, accompanied by better feed conversion and nutrient digestibility. Probiotic supplementation also enhanced hepatic vitamin accumulation. In the Cellobacterin-T group, vitamin A increased by 15.1%, E by 22.8%, B₂ by 19.0%, B₁₂ by 27.2%, and total carotenoids by 21.4% (P<0.01). These findings indicate that probiotics promote beneficial microbiota development, improve nutrient assimilation, and enhance productivity and antioxidant status in broilers. The use of Cellobacterin-T and ProbioFerm-D is therefore recommended as an effective approach to optimize gut microbiome balance in poultry production.
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