THE INFLUENCE OF THE PEGYLATED ANTIBIOTIC ENROFLOXACIN ON BLOOD PROTEIN CONTENT AND LIVER STRUCTURE OF RATS
DOI:
https://doi.org/10.37000/abbsl.2024.110.21Keywords:
rats, pegylated antibiotic enrofloxacin, PEG-400, enrofloxacin, total protein, protein fractions, liver microstructure.Abstract
Among medicines, antibacterial drugs have the most distinct side reactions, in particular, the antibiotic enrofloxacin can cause a toxic effect on the body. The synthesis of new compounds of the antibiotic enrofloxacin with improved therapeutic efficacy and minimal side effects is relevant. The aim of the work is to investigate the content of blood serum proteins and the structure of the liver of rats after intramuscular injections of the pegylated antibiotic enrofloxacin and the drugs that were used in its creation - the traditional form of the antibiotic enrofloxacin and the PEG-400 polymer. For four days, daily, once a day the control animals were injected intramuscularly with a saline solution, and the experimental groups were given the antibiotic enrofloxacin in a traditional substance (the first group), the polymer PEG-400 (the second group) and the pegylated antibiotic enrofloxacin (the third one). On the 7th, 14th, and 21st days after the end of drug injections, blood and liver samples were taken from the animals for research. Total protein and its fractions - albumin, alpha-, beta-, and gamma globulins were tested in blood serum. Liver samples were examined histologically. The conducted studies showed that intramuscular injections of the pegylated antibiotic enrofloxacin, PEG-400 polymer and traditional antibiotic enrofloxacin to rats had little effect on the total protein content in the blood serum of rats. The albumin content in the blood of animals treated with the pegylated antibiotic enrofloxacin, compared to control and other experimental animals, remained at a physiologically higher level, which indicates a stable protein synthesizing function of the liver. During the first 7 days after the end of drug treatment, the content of alpha-globulins in the blood serum of experimental group of rats was lower, and highly dispersed globulins (beta- and gamma-globulins) differed little from the control ones. This may indicate a slight effect of drugs on the cells of the reticulohistiocytic system, which are involved in the formation of beta and gamma globulins. Histological studies of liver tissues showed that pegylation of the antibiotic enrofloxacin reduced hepatotoxicity, since changes in the structure of hepatocytes were recorded only in the first 7 days of its use, and morphological disorders of the parenchyma were detected on the 7th and 14th days after the injection of the traditional form of the antibiotic enrofloxacin and 21 days after giving injections. Therefore, pegylation of the antibiotic enrofloxacin leads to a decrease of its toxic effect on the body, in particular, on the liver after intramuscular injections.
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