THE EFFECT OF INTRAOVULAR ADMINISTRATION OF SILVER NANOPARTICLES (AgNP) ON EMBRYONIC DEVELOPMENT, PRODUCTIVITY, ANTIOXIDANT, AND IMMUNE STATUS OF BROILERS
Keywords:
silver nanoparticles; AgNP; intra-egg administration; broilers; hatchability; growth; antioxidant status; immunity; HSP70; lipid metabolism; poultry farmingAbstract
Abstract. This study investigated the dose-dependent effects of intra-egg administration of silver nanoparticles (AgNP) on embryonic development, hatchability, growth, as well as the physiological, biochemical, antioxidant, and immune status of broilers. The experiment was conducted using different concentrations of AgNP, followed by an assessment of productive and metabolic parameters in the postnatal period. It was found that AgNP contribute to increased hatchability and reduced embryonic mortality. The best growth parameters (body weight, weight gain, feed conversion ratio) were observed at a dose of 15 mg/L. The study also revealed a positive effect of silver nanoparticles on hematological and biochemical blood parameters, specifically increased levels of hemoglobin, hematocrit, and growth hormone. In addition, AgNPs improved the serum lipid profile (decreased LDL-C and triglycerides, increased HDL-C), activated the antioxidant system (SOD, catalase, total antioxidant activity), and increased the expression of the heat shock protein HSP70. Immunological parameters also showed positive changes, manifested by increased lysozyme activity and cytokine levels (IL-1β, IFN-γ, IL-10, C3). The results obtained indicate a pronounced biostimulating effect of AgNPs at optimal doses and their potential to improve productivity and stress resistance in broilers in poultry farming. At the same time, a dose-dependent nature of the effect was established, which requires further optimization of nanoparticle application.
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