STRESS PROTEIN SYNTHESIS IN MUSSELS MYTILUS EDULIS
DOI:
https://doi.org/10.37000/abbsl.2020.97.08Keywords:
mussels Mytilus edulis, stress proteinsAbstract
Mussels 3-6 cm in length were acclimatized to aquarial conditions for a month. Under normal physiological conditions (pure sea water), a set of polypeptides in the range of molecular weights from 10 to 92 kD is synthesized in mussel tissues. Polypeptide with a molecular weight of 68 kD is present in trace amounts. Drilling mud at a concentration of 0.0001% did not lead to significant changes in the electrophoretic spectrum of proteins compared with that under normal physiological conditions. Increasing the concentration of drilling mud to 0.0005% led to changes in the electrophoretic spectrum of proteins. The content of the component with a molecular weight of 68 kD increased significantly. Also increased the relative content of components with molecular weights of 40 and 55 kD, decreased - components with molecular weights of 45-46 kD, as well as in the range of 10-38 kD. Some polypeptides with molecular weights of 15-17 kD and 32-33 kD have disappeared, and some with molecular weights of 34-35 kD have appeared. The relative content of the component with a molecular weight of 48 kD remained the same in all cases, which may indicate the constitutive nature of the synthesis of this protein. The electrophoretic spectra of polypeptides synthesized in vitro when used as a template mRNA from mussel tissues in a wheat-germ and rabbit-reticulocyte lysate cell-free systems were similar. Protein with a molecular weight of 68 kD was detected among the translation products of mRNA from mussels, which were under physiological conditions and under the influence of drilling mud in concentrations of 0.0001% and 0.0005%. At the same time, in vivo, the relative intensity of this component increased significantly only under the influence of drilling mud at a concentration of 0.0005%. In all in vitro mRNA translation products, the relative content of components with molecular weights of 35, 42, 53-55 kD increases in comparison with those in vivo, and the relative content of the component with molecular weight of 32 kD decreases. These data suggest that the mussels Mytilus edulis have constitutive and inducible heat shock proteins. The results also suggest that genes encoding heat shock proteins with a molecular weight of 68 kD are transcribed into mussels under normal physiological conditions and under the influence of drilling mud at a concentration of 0.0001%. However, a small amount of this protein is synthesized in vivo under these conditions. When increasing the concentration of drilling mud to 0.0005%, the intensity of synthesis of this component increases significantly. That is, this protein is synthesized constitutively, and its synthesis increases significantly with increasing concentration of drilling mud. Thus, the protein with a molecular weight of 68 kD can be used as a biomarker of seawater contamination by lignosulfonate drilling muds in ultra-low concentrations.
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